Chemical heater-based, instrumentation-free molecular detection of
Download scientific diagram | Chemical heater-based, instrumentation-free molecular detection of SARS-Cov-2 in saliva. (A) Test workflow: (1) collect saliva sample in a tube; (2) mix saliva with lysis buffer; (3) aliquot the sample to individual tubes, each dry-storing an RT-LAMP reaction mix specific to the selected target; (4) insert tube(s) in the chemical heater and add water to the EPCM to initiate an exothermic reaction and heat the tubes to 60-65 °C for ∼30 minutes. (B) Color change of the tube indicates whether the test is positive (yellow, three tubes) or negative (red, one tube). Here, the negative test is a negative control to verify that the color change does not occur because of the chemical composition of the sample. from publication: Electricity-free chemical heater for isothermal nucleic acid amplification with applications in COVID-19 home testing | Molecular detection of pathogenic nucleic acids from patient samples requires incubating biochemical reactions at specific temperatures to amplify DNA. This incubation is typically carried out with an electrical heater and a temperature controller. To reduce test cost, to | COVID-19, Nucleic Acids and Nucleic Acid Amplification Techniques | ResearchGate, the professional network for scientists.
Thermal Study of Thin-Film Heater for PCR Reaction-Based Applications
Ruijie LI, Associate Researcher, PhD, Chinese Academy of Sciences, Beijing, CAS, Technical Institute of Physics and Chemistry
Reverse transcription lesion-induced DNA amplification: An instrument-free isothermal method to detect RNA - ScienceDirect
A) Schematic illustration of the RPA process. (B) Models of SHERLOcK
Application of Taguchi's method to optimize the reaction conditions
Sensors, Free Full-Text
Ruijie LI, Associate Researcher, PhD, Chinese Academy of Sciences, Beijing, CAS, Technical Institute of Physics and Chemistry
Electricity-free chemical heater for isothermal nucleic acid amplification with applications in COVID-19 home testing - Analyst (RSC Publishing) DOI:10.1039/D1AN00309G
Multistage Chemical Heating for Instrument-Free Biosensing
Molecules, Free Full-Text
Mean difference in the viral load (log10) of HBV DNA, HCV RNA and HIV-1
Frontiers Europan Molecular Indicators of Life Investigation (EMILI) for a Future Europa Lander Mission
