TNE Buffer  Quality Biological

TNE Buffer Quality Biological

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TNE Buffer is often used as a base material to generate lysis buffers. Contains Tris Base (10mM), NaCl (100mM), and EDTA (1mM) Filtered through a 0.22 µm membrane DNase, RNase, and Protease tested Applications TNE Buffer is useful for the isolation of proteins from yeast cells as a lysis buffer, resuspension of pelleted virus or reporter virus particles and the fluorometric determination of DNA content. Recommendation Without correcting the pH, concentrated virus can be used in non-denaturing SDS/PAGE-Western Blot analysis. If the pH is corrected to inhibit triggering of the viral fusion machinery (for West Nile Virus, this corresponds to pH 7.4), the concentrated virus will remain infectious.   Storage Conditions: 15°C to 30°C Shipping Condition: ambient temperature Sterile Filtered pH: 8.0 ± 0.02 Max Shelf Life: 36 months from date of manufacture Harmonization Code: 3822.00.5090 ECCN: EAR99  

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